A Structural Gene Encoding β-Amylase of Barley
نویسندگان
چکیده
منابع مشابه
Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley β-amylase.
There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in germinating barley grains. As a precursor to in vivo studies, here we report syst...
متن کاملGibberellin-Responsive Elements in the Promoter of a Barley High-pl a-Amylase Gene
Deletion analysis has previously shown that the major gibberellic acid (GA)and abscisic acid (ABA)-responsive elements in the promoter of a high-pl a-amylase gene of barley are located downstream of -174 (Jacobsen and Close, 1991). We have used transient expression assays in barley aleurone protoplasts to identify sequences between -174 and +53 that confer GA and ABA responsiveness on expressio...
متن کاملGibberellin-responsive elements in the promoter of a barley high-pI alpha-amylase gene.
Deletion analysis has previously shown that the major gibberellic acid (GA)- and abscisic acid (ABA)-responsive elements in the promoter of a high-pI alpha-amylase gene of barley are located downstream of -174 (Jacobsen and Close, 1991). We have used transient expression assays in barley aleurone protoplasts to identify sequences between -174 and +53 that confer GA and ABA responsiveness on exp...
متن کاملIMMUNOHISTOCHEMTCAL LOCALIZATION OF a-AMYLASE IN BARLEY ALEURONE CELLS
a-Amylase was localized in aleurone cells of barley using immunohistochemical methods. Anti-a-amylase antibody was produced by rabbits immunized with enzyme purified from malt diastase and Himalaya variety barley seeds. Immunoelectrophoresis showed that the antibodies to both antigens were immunologically similar, therefore, they were used interchangeably in the localization of a-amylase. Fluor...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1995
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.59.1991